Catalog number: X1854B
Name: SIAH1 antibody E3 Ubiquitin Ligase - Blocking Peptide
Price: 168.00 EUR
Size: 50 μg
Category: Proteins & Peptides
Long description: Seven in absentia homolog 1 (SIAH-1) is a member of the RING-finger-containing E3 ubiquitin ligases. Alpha-synuclein and synphilin-1 are substrates of SIAH-1 , both proteins are involved in the development of Parkinson's disease (PD). Mutations in Parkin, another E3 ubiquitin ligase which ubiquinates synphilin-1 and glycosylated alpha-synuclein, have been defined as a major cause of autosomal recessive PD. The role of SIAH-1 in PD is highlighted by the fact that SIAH-1 is a component of the Lewy bodies and plays a role in apoptosis caused by nitric oxide (NO) induced oxidative stress. _x000B__x000B_Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) a classic glycolytic enzyme, and multi-functional protein. GAPDH plays role as a mediator for cell death. GAPDH translocates to the nucleus under a variety of stressors/conditions, most of which are associated with oxidative stress. Sequential steps lead to nuclear translocation of GAPDH during cell death; 1] a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) which is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); 2] the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it;3] the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. _x000B__x000B_GAPDH may be genetically associated with late-onset Alzheimer's disease. (-)-deprenyl, which has been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and has neuroprotective actions. The molecular action of this protection is as yet not clear.
Antibody come from: n/a
Other description: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
Clone: not specified
Antigen-antibody binding interaction: SIAH1 antibody E3 Ubiquitin Ligase - Blocking Peptide
Antibody is raised in: see techfile
Antibody's reacts with: see techfile
Antibody's reacts with these species: This antibody doesn't cross react with other species
Antibody's specificity: No Data Available
Application: Antibody function blocking
Antibody's suited for: Blocking peptide for use with Trag-3 antibodies (Cat. Nos. X1871P & X1872P).
Storage: -20ºC
Relevant references: no information yet
Protein number: see ncbi
Warnings: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Properties: If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.blocking peptide
Test: You can block the antibody by the specific target amino acid sequence of peptide.
Description: Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
French translation: anticorps